Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B75000-100.0.JPG-250.jpg)
Research Products International Corp bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane], 100 Grams
Substance Name: bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane
CAS Number: 6976-37-0
Molecular Formula: C8H19NO5
Molecular Weight:?209
Chemical Purity:?>99%
Soluble in: Water
Store at:?Room Temperature
Bioworld TBS Buffer, High Salt (10X), 4 L
Product DescriptionTris-buffered saline (TBS) is high salt used for Western blot washing with a concentration of 10X. It contains 0.5M Tris, 4.5M NaCl and has a pH level of 7.4. Buffer is stored at room temperature.TBS is isotonic and non-toxic, and is used to dilute substances. It is used as a washing buffer for electrodes and to wash microtiter plates for Western blot experiments and screening methods.
Alkali Scientific CellPro™ Tris Buffer-HCL, 1M, pH 8.0, 20L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Sigma Aldrich Fine Chemicals Biosciences Piperazine | 110-85-0 | MFCD00005953 | 100 g
Piperazine | Purity: ≥99.0% | Mol Wt: 86.14 | 110-85-0 | MFCD00005953 | 100 g
Biotang Inc TRIS-HCL Buffer 1.0M, pH 6.8 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 6.8 (0.2 micron filtered), 500 ml.
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS Propane | 64431-96-5 | MFCD00004689 | 100g
BIS-TRIS Propane | Purity: 99% | Mol Wt: 282.33 | 64431-96-5 | MFCD00004689 | 100g
eMolecules 610769-36-3 | TAE buffer | MFCD00236357 | 25g
Combi-Blocks, Inc. | TAE buffer | 25g | 586078156 | QF-5924 | 95.000 | 610769-36-3 | MFCD00236357 | 473.432 | C16H31N3O13
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Alkali Scientific CellPro™ Tris Buffered Saline 10X, pH 7.4, 500mL
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.